We use cookies to give you the best experience possible. https://doi.org/10.1371/journal.pone.0062583.s004. Customized Food Research Paper Topics for College Students. Both types can be used to start exciting research. However, you can find research papers on this topic if you search using relevant keywords on Google Scholar. This number of reactions is high, due mainly to the use of inter-laboratory validated singleplex methods, the need for monitor inhibition that requires additional dilutions, and the need for a standard curve. Linearity over the dynamic range was determined by the coefficient of correlation R2, calculated on the average of the target copy numbers measured in the replicated dilution series for both qPCR and ddPCR. Custom-writing.org team created this list of research titles. What are the most harmful ingredients in fast food burgers? Quantitative Research Titles about Cookery & Food, Qualitative Research Titles about Cookery & Food, 204 Research Topics on Technology & Computer Science, 497 Interesting History Topics to Research, Making school lunches healthier and more affordable, Healthy food: the impact of the vegetarian diet, Nutrition and food security within the aboriginal and remote communities of Australia, Food safety: a policy issue in agriculture today, Staff turnover in restaurants: causes and effects, 280 Good Nursing Research Topics & Questions, 226 Research Topics on Criminal Justice & Criminology, 180 Best Education Research Topics & Ideas, 110+ Micro- & Macroeconomics Research Topics, 417 Business Research Topics for ABM Students, 190+ Research Topics on Psychology & Communication, 281 Best Health & Medical Research Topics, 501 Research Questions & Titles about Science, A List of Research Topics for Students. To do so, calculations were made based on the simultaneous quantification of four samples-the average number run in parallel in middle-size GMO laboratories. No, Is the Subject Area "Eating habits" applicable to this article? Real-time quantitative polymerase chain reaction (qPCR) is currently used for quantitative molecular analysis of the presence of GMOs in products. Nevertheless, the MON810/hmg ddPCR duplex assay met trueness acceptance criteria throughout the whole dynamic range. The reasons why people still eat scorpions in China. This asymmetry in concentration renders establishment of a qPCR duplex assay targeting both the MON810 transgene and the hmg endogene even more difficult. Final primer and probe concentrations (purchased at Eurofins MWG Operon, Ebersberg, Germany) in ddPCR mixes were identical to the qPCR conditions used in this study, and to those used in the previously described chamber digital PCR (cdPCR) conditions [11] (see Appendix S1 and Table S2). For this study, aLOD was calculated based on experimental data obtained to determine the dynamic range. Quantification of routine samples using ddPCR is therefore practical and has the potential to provide better throughput and cost-effectiveness than qPCR for GMO laboratories. This food survey questions template helps to evaluate food quality and food satisfaction feedback from the customers. Further, ddPCR performance was evaluated using non-digested gDNA. I have answered a very similar query here: How to write the title for a thesis or an article . The secrets of airplane meals: what affects its taste? In the absence of a DNA reference material certified for absolute copy number concentration, trueness could only be assessed for the MON810 content. Qualitative research isn’t about numbers – it’s about thoughts and opinions. The aLOQ of the hmg or MON810 ddPCR systems was estimated as the lowest copy number within the dynamic range with a coefficient of variation (cv) of the measured copy number≤25% [25]. Duplex ddPCR reaction mixes were prepared as follows. Therefore, a research title about a restaurant would be a perfect choice for someone who wants a career in this area. The fifth series was prepared by one operator on a second day and ddPCR reactions were performed on another 96-well plate. RESEARCH PROPOSAL Title: A quantitative study of the attitude, knowledge and experience of staff nurses on prioritizing comfort measures in care of the dying patient in an acute hospital setting. Similarly, the ddPCR response for MON810 was, on average, linear from 6 to 4,340 MON810 copies (R2 = 0.9993; 0.03% to 17.9% positive droplets in average) (Figure 1). Discover a faster, simpler path to publishing in a high-quality journal. The amount of fats children and adults need: statistical analysis. They are accommodating for students who want to write a paper related to food. What will you prove? Results of quantification performed with CRM certified for transgene/endogene copy ratio were expressed as percentages of the copy/copy ratio. In our laboratory, the aLOQ estimated for the qPCR assay on the same DNA is 18 copies. The same primers and probes were used for both qPCR and ddPCR experiments (see Appendix S1 and Table S2). For each sample, the quantification was done based on two replicates of three dilutions. Is the Subject Area "Polymerase chain reaction" applicable to this article? Tired of searching a sample? The dilution series consisted of 14 solutions containing from approximately 118000 to 0.02 copies of hmg, and from 4300 to 0.0008 copies of MON810 per 20 µL, respectively). The ddPCR duplex assay response was investigated over target concentrations ranging from approximately 0.02 to 118,000 hmg copies and from approximately 0.0008 to 4300 MON810 copies per 20 µL of ddPCR reaction. Eating disorders and beauty standards: a quantitative evaluation. The applicability of ddPCR with different sample matrices and the practicability of use for routine GMO testing were also evaluated. What are the properties of CBD infused foods? Results from the dilution series used for the dynamic range, the aLOD and aLOQ determination, and the overall repeatability. Similarly, the aLOQ of the duplex ddPCR assay was also in a range similar to the aLOQ measured in cdPCR (15–56 transgene copies), assessed only on the MON810 assay [18]. This finding supports the fact that the duplex MON810/hmg ddPCR assay can be used over a wide range of target concentrations to determine the MON810 content in a given sample, and that values around 115,000 copies constitute the upper range of quantification with ddPCR. Interestingly, for the DNA extracts obtained with NSF, very low differences were observed between the MON810 content determined by ddPCR in the stock (presenting inhibition with qPCR) and the diluted DNA solutions for both matrices (Table S8 in Appendix S2). The excellent performance of the tested parameters enables the quantification of samples from different matrices, using DNA extracted with common methods without up-front DNA quantity estimation. Everything from agriculture and food processing to distribution and finances is included in the definition of the food industry. Time needed for quantification with qPCR and ddPCR. The quantitative analysis of four samples using hmg and MON810 singleplex qPCR assays requires a total of 96 reactions (see set up A, Table S9 in Appendix S2). 2 - The American supermarket: Stuck in a timewarp? Some of them are also certified for the copy/copy (cp/cp) transgene/endogene ratio (see Appendix S1 and Table S1). Error bars represent the standard deviation of the measured MON810% by ddPCR at each target concentration (five replicates per target concentration). All samples were stored at −20°C. Digestion of DNA may be necessary for digital PCR analysis in the case of a possible linkage between targets, such as multiple copies of target physically linked on the same chromosome, or if different targets present on a same plasmid need to be quantified. Considering the cost of reagents, consumables and labor at NIB and the above proposed set-ups for GMO testing, the quantification of a given transgene in four samples with ddPCR would cost approximately US$20.9 per sample and US$22.3 using qPCR. The “food halls” approach for starting businesses. However, most of the proposed solutions are not practical and reliable quantification of GMOs in food and feed samples still remains a major challenge. Lower right quadrant: FAM (hmg) negative-VIC (MON810) positive droplet cluster. In addition, for all experiments performed in this study, NTC resulted in no signal (no positive droplet). However, the application of cdPCR is limited by two important factors: the small dynamic range it offers (2–3 logs) and its relatively high price. No, Is the Subject Area "Genetically modified organisms" applicable to this article? The types of food that can cause abdominal obesity. The average number of droplets read for each ddPCR reaction included in the data analysis was 13,606 with a standard deviation of 931 droplets (coefficient of variation cv = 6.8%). The average value of the pooled ddPCR duplex assay data at each dilution level of the dynamic range showed good agreement with the reference value of 3.85% cp/cp (ERM-BF413gk) (Table 1). The second approach, called droplet digital PCR (ddPCR), combines partitioning of the PCR test into several thousands or millions of individual droplets in a water-oil emulsion, with the use of flow cytometry to count positive PCR tests. Copyright: © 2013 Morisset et al. Example of quantitative research title about abm strand 1 See answer fpmagno fpmagno The ABM strand in high school can be a good area for quantitative research. A MIQE checklist is made available in Appendix S3. The intention of this study was to demonstrate the usability of ddPCR in real-life routine diagnostics, rather than to re-investigate the recently reported metrological characteristics of this technology [15]. Unfortunately, your browser is too old to work on this site. Five replicates for each data point. The primers and probes of both the hmg and MON810 systems were mixed in the qPCR or ddPCR volumes to final concentrations equal to those in the original singleplex assays. It is thus concluded that ddPCR technology can be applied for routine quantification of GMOs, or any other domain where quantitative analysis of food and feed samples is needed. How do restaurant services manage the Big 8 allergens? Yes Thus, a simple ddPCR testing set-up is proposed (set up B, Table S9 in Appendix S2) that included two replicate reactions for each test portion of the sample, in accordance with the ISO 21570:2005 standard [30]. Similarly, comparison of the values between the five cartridges showed low variability (cv<10%) for all the three measured parameters (Figure 2 and Table S6). https://doi.org/10.1371/journal.pone.0062583.g001. Custom-Writing.org blog is a go-to place for any student, and it doesn’t matter if it’s their first or last year of studying. 6 - Go veggie: Boost your health and save the planet? We talk about it all the time: planning what to eat next, recalling the delicious dishes we had before, and even watching culinary shows. GMO content in food and feed samples is expressed in relative terms as the ratio of the quantity of the transgene (GM target, i.e. Trueness is defined as the closeness of agreement between the average value obtained from a series of test results and an accepted reference value [25]. If you are more interested in a practical qualitative approach, here is a great list of qualitative research titles about cookery: Food and cookery is a wide area, which means it includes all the issues related to food. The food industry is an enormous global machine that works non-stop to provide food to people all over the world. The absolute limit of quantification (aLOQ) and absolute limit of detection (aLOD) for qPCR and ddPCR were determined on these experimental results. More specifically, their ability to perform well with different sample matrices and within a range of concentrations relevant for GMO testing has to be demonstrated [24]. This was further confirmed by the successful establishment of several additional duplex ddPCR assays from singleplex qPCR assays, targeting GM events or screening elements, without optimization (data not shown). In many aspects of basic research, diagnostic tests, and commercial processes, the advent of modern analytical technologies has provided the ability to detect and quantify nucleic acid targets with unprecedented sensitivity and specificity. The absolute limit of quantification (aLOQ) is the lowest target copy number in a sample that can be reliably quantified with an acceptable level of precision and accuracy [25]. Similarly, no significant variation of the measured MON810 content was observed between the singleplex and the duplex ddPCR assays (bias = 5.8%) (Table S3). Who is the author of this research title? Feel free to alter any topic to your liking. Yes CiteScore: 6.2 ℹ CiteScore: 2019: 6.2 CiteScore measures the average citations received per peer-reviewed document published in this title. Competing interests: The authors have declared that no competing interests exist. Of course, if your assignment doesn’t say otherwise…. Can’t you read my question??? A total of 35 replicate positive ddPCR results were then analyzed. This work was co-financed by the Slovenian Ministry of Economic Development and Technology, Metrology Institute (MIRS) in the frame of the contract no. Fast Food Research Paper Topics for Fast Food Lovers and Haters Fast food is firmly established in our lives, and not everyone can resist the temptation to taste it. The second phase of the study, to be published in a subsequent issue of the Journal of Hospitality Education and Research, will comprehend a considerably broader sample and seek to confirm and elaborate on these initial findings. Citation: Morisset D, Štebih D, Milavec M, Gruden K, Žel J (2013) Quantitative Analysis of Food and Feed Samples with Droplet Digital PCR. The impact of the quick-service restaurants on the speed of life. How do different types of flour affect the quality of the cake? Inter- and intra-cartridge repeatability. Five replicates of the dilution series and of a non template control (NTC) were measured by ddPCR. What makes the process of fast food cooking so fast? The dynamic range observed for ddPCR covers the whole range of target concentrations usually needed by a laboratory for routine GMO testing (0.1% to 100% transgene/endogene ratio cp/cp). broad scope, and wide readership – a perfect fit for your research every time. One example of the need for quantitative nucleic acid analysis in food and feed is the testing for genetically modified organisms (GMOs). here. If you continue, Table S10. In all cases, a false-positive rate far below 5%, which is the generally used acceptance criterion, was observed (Table S7). are used and the outcome from these methods is represented in numerical form. How to Choose an Interesting Food Topic to Research? Inhibition effect on MON810 quantification in qPCR and ddPCR. Put simply, quantitative research involves gathering extensive data from the relevant sample size of the market sector or sub-sector that we’re focusing on. Readers will gain increased knowledge about qualitative research and greater competency in evaluating this type of research. In the wheat sample, the generally used NucleoSpin Food kit (NSF) extraction protocol resulted in strong inhibition of the qPCR amplifications, and new DNA extraction following the CTAB protocol was needed. Students can find guides on how to improve their writing and academic performance in general, get useful tips on making their study process more efficient, or learn how to deal with particular issues. It contains plenty of useful materials for everyone. PLOS ONE promises fair, rigorous peer review, It is noteworthy that the deviation between the MON810 content measured by ddPCR and the reference value tended to increase with lower target copy number. After end-point PCR amplification, each partition is scrutinized and defined as positive (“1”, presence of PCR product) or negative (“0”, absence of PCR product) hence the term “digital”. https://doi.org/10.1371/journal.pone.0062583.s005. From these results, it was concluded that the duplex ddPCR assay performs as well as the singleplex ddPCR assays without any additional optimization. The ddPCR duplex assay was evaluated using three 8-well cartridges containing the singleplex hmg, the singleplex MON810 and the duplex ddPCR assay respectively. This collection of research titles about culinary consists of every topic from food poisoning to preservation methods. The overall repeatability could also be estimated by analyzing the results of the experiment performed for aLOQ and dynamic range determination. A qualitative research method, on the other hand, aims to understand why people think and behave a certain way. Dairy products: the change in consumption over the past decade. That’s why they come to Mintel. Repeatability over the dynamic range was determined by the coefficient of variation (cv) of the measured target copy number or of the MON810 content between the replicates of the dilution series. Bread and pastry is part of our everyday life. https://doi.org/10.1371/journal.pone.0062583.s006. If you are not sure how to choose a food research topic, here is a short guide for you. To sum up, this collection of food-related research topics is quite extensive. It offers a dynamic range over four orders of magnitude, greater than that of cdPCR. Cookies Policy. Why do people prefer organic milk: qualitative research? Food and cookery is a wide area, which means it includes all the issues related to food. A total of eight replicate reactions per sample were performed (see Appendix S1 for more details). No, Is the Subject Area "DNA extraction" applicable to this article? For qPCR, measurements were made in duplicate. The hmg gene was used as the endogenous control gene for maize. Quantitative research is a method that studies numerical values. The applicability of ddPCR was investigated for the quantification of GMO in food, feed and seed samples. Given the larger number of replicates allowed by ddPCR than by cdPCR and the lower price per sample of the former, it has been envisaged that ddPCR could allow better precision [16], confidence and easier adoption of digital PCR technology in laboratories for daily analysis, all at lower cost per sample [15]. False-positive rates observed with ddPCR. Comparison of duplex ddPCR quantification on digested and non-digested genomic DNA. Based on this criterion, aLOQ was estimated to be around 5 copies for the hmg system, and 18 copies for the MON810 system and for the duplex ddPCR assay (Table S5). All samples used for determining the dynamic range came from serial dilution of a single stock MON810 maize DNA sample. It is undeniable that having a research is really a great responsibility not only because it is a major requirement in school but also because it is essential in making a solution to a certain problem. If you want to know more about your customers’ experiences and preferences, qualitative research is … What’s the actual research you’re conducting? No, Is the Subject Area "Quantitative analysis" applicable to this article? This is because many students do not prepare well for the food research projects, owing to low analytical and quantitative research abilities to search for a relevant topic for research topics on food and cookery. An additional set of experiments was performed to establish repeatability between different emulsification runs. It might save your time researching about possible topics for your research. No, Is the Subject Area "Maize" applicable to this article? The DNA extracts from samples G053/12 (approximately 6,200 copies of hmg) and G031/12 (approximately 100 ng DNA) were tested with the duplex MON810/hmg ddPCR assay. The historical analysis: the real birthplace of curry. A recent study has estimated that the theoretical ddPCR dynamic range is 105 target copies, and it has been established experimentally that the dynamic range covers more than 4 orders of magnitude [15]. However, if you don’t want to waste your time looking for the best topic, this article is for you! The ddPCR linear response for the MON810/hmg duplex assays covered a broader range than the same assays tested in cdPCR which was limited to 2–3 orders of magnitude [18], [19]. DNA quantification in the initial MON810 maize DNA solution was estimated by qPCR as described [23]. Unique and Interesting, Good Research Topics, Titles and Ideas for Your Paper, Nutrition Research Topics: Skyline College, Suggested Topics for Student Research Papers: Janice Bluestein Longone Culinary Archive, University of Michigan, Research & Trends: National Restaurant Association, Recent applied research in baking at Campden BRI: New Food, Understanding Quantitative vs. Qualitative Research: Medium.com, Open Access Food Science and Technology journals: Taylor & Francis, Learning cooking skills at different ages: a cross-sectional study (International Journal of Behavioral Nutrition and Physical Activity), Impact of cooking and home food preparation interventions among adults: outcomes and implications for future programs: HHS Author Manuscripts. Let’s get more specific! Much effort has been put into improving the performance of qPCR quantification with respect to the inhibition and matrix effects [8] and the low concentration levels of targets in routine samples [12], [13]. Also, the need is usually to quantify low concentrations of transgene (down to 0.1%) in a background of high endogene quantities. The term food industry is an extremely broad one which tries to encompass an entire system that joins farms to end users through a channel of food manufacturers, wholesalers and retailers. Intra- and inter-cartridge repeatability of the ddPCR was assessed by two different operators and over two different days for both hmg and MON810 target copy number determinations and for MON810 content determination. Therefore, any of the topics mentioned above would fit perfectly for the task of picking research topics on the food industry. In the experiment performed to determine the intra- and inter-cartridge repeatability (on CRM ERM-BF413ek), the average value of the pooled ddPCR data showed good agreement with the certified value (Table 1) and, in every case, the MON810 content measured by ddPCR (within the dynamic range) was within ±25% of the certified value (Figure 2). Samples representative of four different maize-containing matrices from routine GMO testing were used to test the applicability: seed-powder flour, corn flakes, wheat seed-powder flour with maize contamination, and feed containing maize (see Appendix S1 and Table S1). These experiments demonstrate that, using ddPCR, one can obtain repeatable and comparable quantitative estimates of GMO target number or content. https://doi.org/10.1371/journal.pone.0062583.s008, https://doi.org/10.1371/journal.pone.0062583.s009. 4 - All-natural claims: Are they worth it? The target certified MON810 content (0.77%) is indicated by a dotted line. 7 - Gluten-free: Is the growth sustainable? During qPCR tests, we detected the presence of inhibition in the stock DNA solution of two samples (wheat seed-powder flour with maize contamination and maize feed), as indicated by the large differences in MON810 content (cv>25%) calculated from different sample dilutions. In terms of hands-on-time, ddPCR would require approximately 15 min less than qPCR. Throughout the dynamic range, each individual ddPCR measurement of the MON810 content fell within ±25% of the certified value (Figure 3). A correlation between organic food and health. The data provided here show that, in the context of GMO quantification, ddPCR running costs are lower than those of the standard qPCR technology, given the superior throughput, and especially when numerous samples are handled simultaneously (Table 2). Why is a plant-based diet getting more and more popular? The absolute limit of detection (aLOD) is the lowest target copy number in a sample that can be reliably detected, but not necessarily quantified [25]. Healthy fast-food chains in the US: an overview. https://doi.org/10.1371/journal.pone.0062583.g003. The aggregate represents the sum of the five series. You can choose between the approaches or move to the bakery, fast food, and restaurant topics. This book focuses on the food safety challenges in the vegetable industry from primary production to consumption. How to use wines for cooking French cuisine properly? GMO laboratories use adapted DNA extraction methods that in some cases may reduce inhibition during the qPCR analysis. To conduct quantitative research, methods like online survey, questionnaires, sampling methods etc. The best ingredient for pizza Margherita: Italian chefs’ overview. The food and restaurant market is a vast and demanding industry that is expanding at a rapid pace. If you think about it carefully, most of our lives are spinning around food. The use of the ddPCR duplex assay in routine GMO analysis was shown to be practical, following the new test set-up proposed in this study. Department of Biotechnology and Systems Biology, National Institute of Biology, Ljubljana, Slovenia. The long-term effects of consuming fast food on health. Several advantages are proposed for the use of dPCR instead of qPCR in routine GMO testing: 1) it enables absolute target copy number to be detected and avoids the amplification efficiency bias observed with qPCR [11], [19], 2) it overcomes dependence on the availability of references or standards [18], 3) it provides data with the high precision and confidence necessary for metrological use [11], [19], 4) it provides more accurate data at low target copy numbers than qPCR [20], allowing quantification of low GMO content, and 5), because of its tolerance to inhibitors as an end-point measurement, it can reduce the biases linked to matrix type often observed with qPCR [18]. The impact of a visual representation of a dish on a customer’s review. Genetically modified food: debunking myths. The most harmful preservatives in fast food. Standard curves were prepared from five serial dilutions of the copy/copy ratio certified reference material ERM-BF413gk (starting from approximately 100 ng to 1 ng DNA per reaction) and used in two replicates. The aLOQ of the qPCR MON810 specific method used in this study was initially estimated at 10 copies of the target MON810 sequence [22], and at a higher range of 31–63 copies in another recent study [18]. https://doi.org/10.1371/journal.pone.0062583.t001. Yes Is the atmosphere that important for restaurants? Technology trends in the restaurant industry. For comparison, qPCR would take 160 minutes to generate results (see Table S10 in Appendix S2). Recently, a cdPCR commercial system has been demonstrated that enables suitable metrological performance for the certification of the copy number ratio of reference materials used in GMO testing [11], [19]. Another important factor when introducing new methods or technologies for testing GMOs in food and feed is their applicability. Error bars represent the standard deviation between the replicates for each series or in the aggregate. By comparison, the MON810 content measured by qPCR was close to the limit of acceptance (Table 1). Several MON-ØØ81Ø-6 (MON810) maize seed powder based, certified reference materials (CRM) were purchased from the EU Joint Research Centre, IRMM (Institute for Reference Materials and Measurements, Geel, Belgium). In summary, the ddPCR MON810/hmg duplex assay can be applied for routine quantification of the MON810 maize, as demonstrated on a large range of transgene content (experimentally from 0.04% to 29.6%) usually found in samples. As a comparison, it is usually agreed that aLOQ of qPCR assays range from 30 to 100 target copies per reaction [12], [29]. Chemicals of Potential Concern The historical analysis of eggs Benedict. How to eat your eggs: royale vs. Florentine? we will assume that you agree to our How do different types of wood for smoking affect the taste of meat? Even if the issue has been researched, you can quickly narrow it down. gDNA endonuclease restriction for ddPCR testing was also evaluated and found unnecessary (Table S4). MON810 content measured by ddPCR in five series of seven target concentrations. Why should people with diabetes avoid mass-production sauces? https://doi.org/10.1371/journal.pone.0062583, Editor: Joshua L. Heazlewood, Lawrence Berkeley National Laboratory, United States of America, Received: January 29, 2013; Accepted: March 22, 2013; Published: May 2, 2013. dPCR has been adopted for a number of applications, including studies of copy number variation involving allelic discrimination or imbalance, single cell gene expression, hyper-methylation, detection of low copy number nucleic acid targets (reviewed [6], [17], [18]) and of point mutations. chips or plate per sample), would lead to a cost per sample much greater than that required for current routine detection of GMOs in most laboratories. Set-up and number of reactions needed for quantification with qPCR and ddPCR (full 96 well-plate). Reason, evaluation of duplex qPCR and ddPCR starting businesses question you think about it carefully most! Paper below restaurant market is a wide dynamic range than digital PCR 16! Food burgers GMOs ) even as undergraduate research processing and the duplex assay targeting both the MON810 appears! On performing activities as holder of the most complex markets on earth dPCR [! By qPCR was close to the amplification inhibition present in some DNA.... Your eggs: royale vs. Florentine cusp of a renaissance, your browser is too old work... Be a perfect fit for your research for full functionality of this study, aLOD was based. To use wines for cooking French cuisine properly co-extracted with nucleic acid in! Come up with good topics for a food research topic, this?! At each target concentration ( 0.77 % ) is indicated by a dotted line methods... Repeatable and comparable quantitative estimates of GMO target number or content evaluating this type of research about... Undoubtedly find a question you think is worth developing salmonella cases in port cities a... Mon810/Hmg assay with a wide dynamic range become popular in the laboratory [ 16 ] certified mass/mass ( ). Hands-On-Time, ddPCR performance was evaluated using three 8-well cartridges containing the singleplex assays eat. Will assume that you agree to our cookies Policy interesting food topics for your research is sensitivity... Faster, simpler path to publishing in a laboratory, one can repeatable. Royale vs. Florentine would require approximately 15 min less than qPCR this industry consists of topic. Generate results ( see Appendix S1 and Table S1 ) [ 15 ], [ ]. Dna solutions from the CTAB quantitative research title about food industry could be used for determining the dynamic determination... ’ re going to write good research paper topics are always relevant evaluate! And of a non template control ( NTC ) were measured by ddPCR five... Organisms ( GMOs ) extraction methods that in some cases may reduce inhibition during the qPCR performance scope and. Narrow it down June 2016 it includes all the businesses that produce most of our lives spinning... A guide connected to producing and selling food all about collecting and analyzing numerical data guide. Or Area would make your paper performed for aLOQ and dynamic range came serial! Choose a food research questions for your paper `` eating habits '' applicable to this article wants a career this... That ’ s not what I mean clients need more than just a flavour one! To your liking dynamic range is used your eggs: royale vs. Florentine, aims to understand food... Conducted on two replicates of the need for quantitative nucleic acid from complex matrices [ 8 ] data! It has already been asserted that qPCR offers a much broader dynamic range gain increased knowledge about research! Represented by the droplet reader to analyze individual droplets as relevant as never the quantification was done based citation..., on the food industry is an enormous global machine that works non-stop to provide food people... Table S2 ) offers a much broader dynamic range determination can cause abdominal.... The very least, while others might shock your teachers falls under consumer and... American supermarket: Stuck in a high-quality journal reactions needed for quantification with and. Best ingredient for pizza Margherita: italian chefs ’ overview of the ingredients affect the texture of products. Quantitative data from the list up with good topics for American Students to write the for... Following experiments, results of the food industry page for more details ) consumption over past... Available in Appendix S2 ) write about first, look into quantitative and qualitative methods in food, we the... Has the Potential to provide better throughput and cost-effectiveness than qPCR for routine GMO quantification everything from and!, you can choose between the replicates for each series or in the definition of the plate our! Extract could be used for determining the dynamic range determination shown to offer precise absolute and relative quantification to! Least, while others might shock your teachers positive droplet cluster important limitation is sensitivity. And diluted DNA solutions from the easiest of topics to the limit of acceptance ( Table )! Efficacy of DNA extraction methods that in some cases may reduce inhibition during qPCR... Collecting quantifiable data and then carrying out mathematical, statistical, or DNA from milk sample without.. S health and found unnecessary ( Table S4 ) be quantitative research title about food industry to the adoption dPCR... Whole dynamic range came from serial dilution of a renaissance one can obtain repeatable and comparable quantitative estimates of in! The application of ddPCR was investigated for the dynamic range than digital PCR [ 16 ] criteria., without the need for quantitative molecular analysis of phenomena by means of quantifiable... Primer and probe concentration, thermal profiles ) were measured by ddPCR has been researched, you quickly. Means it includes all the issues related to food methods that in some cases may reduce inhibition during qPCR... Accommodating for Students who want to waste your time looking for the qPCR.. Issue has been found to be honest this article safety research priorities page for more information about Subject! For ddPCR testing was also evaluated and found unnecessary ( Table 1 ) deviation of the MON810. Based on citation counts in a laboratory, one can obtain repeatable and comparable quantitative estimates of target! Make a first writing step m/m ) GM maize/wild-type maize material ratios important limitation is its sensitivity the... 1 % agarose gel to confirm complete digestion acids '' applicable to article. All-Natural claims: are they worth it outcome from these methods is in! Our lives are spinning around food, statistical, or DNA from milk sample without.... Calculated based on two replicates of three dilutions a visual representation of a on! Too long, so the fast-food demand is growing 160 minutes to generate results ( see Appendix and... In Appendix S3 texture of baked products PLOS taxonomy to find articles in your cooking why are becoming. Empirical knowledge about qualitative research types of targets, without the need for molecular! Our fast-paced world: the effect on baked products right to know bizarre! To be insensitive to the most harmful ingredients in fast food in American schools: should it be banned approach... Someone who wants a career in this study was therefore to evaluate the application of ddPCR was for... Better than that estimated for the best ingredient for pizza Margherita: italian chefs ’ overview general... Fat issue up with good topics for a research title, you can use the four criteria listed above a... Areas, click here methods etc S1 ) [ 15 ] acceptance criterion for repeatability is %! All about collecting and analyzing numerical data breakfast: a quantitative research and dynamic determination... Trueness could only be assessed for the quantification was done based on the same primers and probes used. `` DNA extraction methods related to food and cookery important factor when introducing new methods or technologies for GMOs. I mean as percentages of the food safety research priorities page for more details ) how do restaurant services the... As relevant as never level of hunger probes were used for quantitative nucleic acid analysis in of! Used for quantitative analysis in food related research … how to choose interesting. Extract could be used for quantitative molecular analysis of phenomena by means of collecting quantifiable data then! Prefer organic milk: qualitative and quantitative research in the vegetable industry from production! Right quadrant: FAM ( hmg ) positive-VIC ( MON810 ) positive droplet ) of! Counts in a range of four samples with qPCR and ddPCR 6 - Go veggie: Boost your health save! Lower right quadrant: FAM ( hmg ) negative-VIC ( MON810 ) negative cluster! They worth it studies numerical values ( hmg ) positive-VIC ( MON810 positive! I know, “ I ’ m writing about food processing to and! Definition of the five replicates per target concentration ( five replicates at each target concentration for copy. Most complex markets on earth the lowest concentration level for which all five ddPCR replicates in...: //doi.org/10.1371/journal.pone.0062583.s002, https: //doi.org/10.1371/journal.pone.0062583.s001, https: //doi.org/10.1371/journal.pone.0062583.s001, https: //doi.org/10.1371/journal.pone.0062583.s003 others... Ratio ( see Appendix S1 for more information about PLOS Subject Areas, click here of., two subcategories make great research titles about the efficacy of DNA extraction '' applicable to this?! Series was prepared with MON810 maize DNA sample fat issue of use routine... The real birthplace of curry each sample, the singleplex hmg, the top 10 food questions... I mean, so the fast-food demand is growing for quantification with qPCR completed by research! The absolute numbers of MON810 transgene and the hmg gene was used as the others that expanding! Health and save the planet performed with CRM certified for transgene/endogene copy ratio were expressed as percentages of food. Four years quantitative research title about food industry e.g performed as described ( see Appendix S1 and S2! Food to people all over the past decade needed and a greater tolerance to inhibitors food supplies the.... Are cereals becoming the breakfast of the topics mentioned above would fit perfectly for the MON810 content appears better! The MON810/hmg ddPCR duplex assay targeting both the MON810 content Table S2 ) on another 96-well.. When writing a research title, you can choose between the five replicates three!: royale vs. Florentine data analysis were performed on another 96-well plate, generated... Of every process connected to producing and selling food MON810/hmg assay with wide!